FIG. 2.

TCDD reduces transcript abundance of Rspo2 in UGS mesenchyme. C57BL/6J mouse embryos were exposed in utero to vehicle or TCDD (5 μg/kg dam, po) at E15.5 and UGSs were harvested at E16.5. UGSs were sectioned along the sagittal plane and are shown with the urethra on the left pointing down and the bladder on the right. Images are representative of at six litter-independent UGSs per treatment group. Within each panel, the boxed area in the left image is enlarged on the right. The purple stain marks transcript localization as assessed by ISH. Rspo2 transcription was restricted to the mesenchyme (A, B). The arrowhead in (A) points to faint staining in the anterior mesenchyme of the UGS. Exposure to TCDD reduces abundance of Rspo2 RNA.