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. 2014 Nov 3;289(51):35061–35074. doi: 10.1074/jbc.M114.621425

FIGURE 5.

FIGURE 5.

In vitro dimerization of WT, mutant, and revertant RNA transcripts. The leader RNA transcripts (nt 1–368) were heat-denatured and analyzed on a denaturing urea-containing gel to check for integrity (upper panel). Alternatively, the RNAs were allowed to fold and analyzed on nondenaturing TBM gel (middle panel) or TBE gel (lower panel) to separate the different RNA conformations, which are indicated along the gels. M, monomer.