FIGURE 6.
Disrupting interactions with the actin cytoskeleton increases the mobility of CD44. Shown are plots (A–E) of normalized fluorescence intensity versus time after photobleaching a small area of plasma membrane (6.72 × 3.36 μm) in transfected K562 cells expressing the indicated CD44-YFP construct. Cells in A were pretreated with DMSO control, latrunculin B (Lat B), or blebbistatin. FRAP curves from four independent trials with five cells per trial were fitted to a function for monoexponential recovery of fluorescence to derive τ½ and Fmax as reported in Table 3. The data represent the mean. Error bars represent S.E.