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EmbC has an N-terminal signal sequence. EmbC fused with either an N-terminal or C-terminal FLAG tag was expressed in M. tuberculosis recombinants. Expression of tagged EmbC was induced by the addition of anhydrotetracycline (ATc) in three independent transformants. Lysates were probed using α-FLAG tag antibodies. N1–3, N-terminal tagged recombinants; C1–3, C-terminal tagged recombinants; +, 49-kDA N-terminal FLAG-BAPTM positive control for the α-FLAG tag antibody.
