FIGURE 4.

Expression level of EmbC in recombinant strains. RNA was purified from M. tuberculosis strains carrying recombinant alleles of embC in which expression was from the indicated promoter. RNA was extracted, and embC mRNA was measured using RT-qPCR and normalized against sigA. Results are given for each promoter and are expressed as fold change compared with the wild-type strain. Each sample was assayed at least twice. A, promoter variants. B, mutant alleles expressed from the Ag85a promoter. A and B are from independent experiments.