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. 2014 Oct 28;289(51):35172–35181. doi: 10.1074/jbc.M114.583112

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Analysis of LAM from M. smegmatis recombinants. The M. smegmatis embCΔ deletion strain was complemented with the indicated embC_Mtb alleles. LAM was analyzed in wild-type and recombinant strains grown on agar. A, periodic acid-Schiff staining. B, Western blotting using antibody CS-35. Lane 1, wild-type M. smegmatis; lanes 2–9, M. smegmatis embCΔ complemented with wild-type embC_Mtb (lane 2), no allele (lane 3), embCBB_Mtb hybrid (lane 4), embC_Mtb N638A (lane 5), embC_Mtb N652A (lane 6), embC_Mtb Ser-341 truncation (lane 7), embC_Mtb Val-637 truncation (lane 8), and embC_Mtb Leu-717 truncation (lane 9); and lane M, markers. C, Western blotting using antibody CS-35. Lanes 1 and 2, wild-type M. smegmatis; lane 3, wild-type embC_Mtb; lane 4, empty; lane 5, no allele; and lane 6, embC_Mtb D293A. The expected range for migration of LAM and LM is shown for the PAS-stained gel. The Western blot detects only LAM.