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. 2014 Oct 28;289(51):35172–35181. doi: 10.1074/jbc.M114.583112

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Analysis of LAM from M. tuberculosis recombinants carrying mutations in the proline motif. M. tuberculosis strains carrying the indicated embC_Mtb alleles were constructed by gene switching. LAM was analyzed in recombinant strains grown on agar. A, periodic acid-Schiff staining. B, Western blotting using antibody CS-35. M. smegmatis embCΔ is complemented with wild-type embC_Mtb (lane 1), embC_Mtb N638A (lane 2), embC_Mtb N638A (lane 3), embC_Mtb N652A (lane 4), and markers (lane M). The expected range for migration of LAM and LM is shown for the periodic acid-Schiff-stained gel. The Western blot detects only LAM.