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. 2014 Oct 28;289(51):35172–35181. doi: 10.1074/jbc.M114.583112

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Analysis of LAM from M. tuberculosis recombinants in liquid medium. M. tuberculosis strains carrying the indicated embC_Mtb alleles were constructed by gene switching. LAM was analyzed in recombinant strains grown in liquid medium. A, periodic acid-Schiff staining. B, Western blotting using antibody CS-35. C, Western blotting using antibody CS-40. Lane 1, wild-type embC_Mtb; lane 2, embC_Mtb N638A; lane 3, embC_Mtb N652A; lane M, markers. The expected range for migration of LAM and LM is shown for the periodic acid-Schiff-stained gel. The Western blot detects only LAM.