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. 2014 Nov 4;289(51):35193–35204. doi: 10.1074/jbc.M114.586461

FIGURE 1.

FIGURE 1.

Related expression of uPA with MUC1 in various cancer cells. A, expression of MUC1 and uPA mRNA in HCT116/Mock and HCT116/MUC1 cells was examined by microarray analysis. B, cell lysates prepared from HCT116/Mock, HCT116/MUC1, A549/Mock, A549/MUC1, SKOV3/Scr, SKOV3/Si-1, and SKOV3/Si-2 cells were subjected to SDS-PAGE, followed by immunoblotting (IB), and detection with anti-uPA, anti-MUC1-CD, and anti-β-actin antibodies. β-Actin served as a loading control. C, expression of MUC1 and uPA in HCT116/Mock and HCT116/MUC1 cells was detected immunocytochemically using the combinations of mouse anti-MUC1-ND and Alexa Fluor 488-labeled rabbit anti-mouse IgG antibodies (green), and goat anti-uPA and Alexa Fluor 594-labeled rabbit anti-goat IgG antibodies (red). Nuclei were stained with DAPI (blue). Images were taken at ×630 magnification. Scale bar: 25 μm.