FIGURE 5.

Enhancement of uPA transcriptional activity in relation to the elevated occupancy of NF-κB p65 on the uPA promoter. A, HCT116/Mock, HCT116/MUC1, SKOV3/Scr, SKOV3/Si-1, and SKOV3/Si-2 cells were transiently co-transfected with a control reporter vector, the pRL-TK vector, plus an empty vector, the WT/uPA vector or the MutNF-κB/uPA vector. B, HCT116/MUC1 and SKOV3/Scr cells were transiently co-transfected with a control reporter vector, the pRL-TK vector, plus an empty vector, the WT/uPA vector or the MutTCF4/uPA vector. The respective cells co-transfected as described above were cultured for 24 h, and then uPA promoter activities of each cell were analyzed by means of luciferase assays. The values obtained for each reporter vector transfectants were normalized as to an internal control, Renilla luciferase, and the promoter activity of empty vector transfectants was taken as 1 (means ± S.D., n = 3, **, p < 0.01; NS, not significant). WT, wildtype uPA promoter vector transfectants; MutNF-κB, NF-κB binding site-mutated uPA promoter vector transfectants; MutTCF4, TCF4 binding site-mutated uPA promoter vector transfectants.