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. 2014 Oct 24;289(51):35351–35362. doi: 10.1074/jbc.M114.593376

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Klotho, βklotho, and KLPH. a, schematic depicting klotho, βklotho and KLPH with putative acid/bases (gray) and nucleophiles (black) in predicted pockets. b, overexpressed myc/His-tagged klotho, βklotho, KLPH, GBA wild-type, and acid/base-lacking E235G GBA in total COS-7 cells with excess ABP 1 or ABP 2 and purified via His-tag pulldown. Labeling with ABP 1 in the absence/presence of sodium azide (first and second gel), idem with ABP 2 (third and fourth gel), and myc-tagged proteins detected with α-myc (fifth gel).