TABLE 1.
Primers employed for the site-directed mutagenesis of GBA, GBA2, GBA3, and LPH
| Coding sequence | Substitution | Direction | Primer sequence (5′ → 3′) |
|---|---|---|---|
| GBA | E235G | Forward | GACAGCTGAAAATGGGCCTTCTGCTGGGC |
| Reverse | GCCCAGCAGAAGGCCCATTTTCAGCTGTC | ||
| E235Q | Forward | GACAGCTGAAAATCAGCCTTCTGCTGGGC | |
| Reverse | GCCCAGCAGAAGGCTGATTTTCAGCTGTC | ||
| E340G | Forward | GCTCTTTGCCTCAGGGGCCTGTGTGGGCTCC | |
| Reverse | GGAGCCCACACAGGCCCCTGAGGCAAAGAGC | ||
| E340Q | Forward | GCTCTTTGCCTCACAGGCCTGTGTGGGCTCC | |
| Reverse | GGAGCCCACACAGGCCTGTGAGGCAAAGAGC | ||
| GBA2 | E527G | Forward | GATTTGGCTACCTTGGGGGCCAGGAGTACCG |
| Reverse | CGGTACTCCTGGCCCCCAAGGTAGCCAAATC | ||
| E659G | Forward | CTGAAATGAAGTTTGGCAAGGACCATGATGGAC | |
| Reverse | GTCCATCATGGTCCTTGCCAAACTTCATTTCAG | ||
| D633G | Forward | GTTTGACAAGGACCATGGTGGACTCATTGAAAATGG | |
| Reverse | CCATTTTCAATGAGTCCACCATGGTCCTTGTCAAAC | ||
| E667G | Forward | CATGATGGACTCATTGGAAATGGAGGCTATGCAG | |
| Reverse | CTGCATAGCCTCCATTTCCAATGAGTCCATCATG | ||
| D673G | Forward | GGAGGCTATGCAGGCCAGACCTATGATGG | |
| Reverse | CCATCATAGGTCTGGCCTGCATAGCCTCC | ||
| D677G | Forward | GACCAGACCTATGGTGGATGGGTGACCAC | |
| Reverse | GTGGTCACCCATCCACCATAGGTCTGGTC | ||
| GBA3 | E165G | Forward | CAGTGGATCACCATAAATGGAGCTAATGTTCTTTCTGTG |
| Reverse | CACAGAAAGAACATTAGCTCCATTTATGGTGATCCACTG | ||
| E373G | Forward | CTGTAATTTACATCACTGGGAATGGGTTTCCCCAG | |
| Reverse | CTGGGGAAACCCATTCCCAGTGATGTAAATTACAG | ||
| LPH | E1065G | Forward | GGATGACTTTTAATGGGCCCATGTACCTGGC |
| Reverse | GCCAGGTACATGGGCCCATTAAAAGTCATCC | ||
| E1273G | Forward | CCATTTACATCACCGGAAACGGAGTGGGGC | |
| Reverse | GCCCCACTCCGTTTCCGGTGATGTAAATGG | ||
| E1538G | Forward | GGATCACGCTGAATGGGCCCTTTGTCATTGC | |
| Reverse | GCAATGACAAAGGGCCCATTCAGCGTGATCC | ||
| E1749G | Forward | CCAATTTATGTCACAGGGAATGGAGTGTCCCAGC | |
| Reverse | GCTGGGACACTCCATTCCCTGTGACATAAATTGG |