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. 2014 Oct 24;289(51):35388–35396. doi: 10.1074/jbc.M114.614297

FIGURE 3.

FIGURE 3.

HDX of the 170-loop. A, HDX plot of peptide 316–325 of the 170-loop. B, localized difference in deuterium uptake for individual residues (red bars) or segments (orange bar) of peptide 316–325 from unbound versus TF-bound FVIIa after 15 s exchange time. C, structural representation of the 170-loop with individual sites shown by ETD to undergo reduced HDX upon TF binding highlighted. Based on HDX-ETD data in B, H-bonds involving amide hydrogens from residues Ile-323 and Glu-325 are likely to be destabilized or absent in unbound FVIIa.