FIGURE 8.

HDX of the interdomain linkage. HDX plots of (A) peptide 133–141 and (B) peptide 134–141 of the interdomain linkage region that contain the S-S bond that connects the light and heavy chain of FVIIa. C, structural representation of the interdomain linkage. Individual sites that are likely to undergo reduced HDX upon TF binding are localized by ETD to residues Ile-138-Leu-141 (PDB ID: 1DAN). The H-bond involving amide hydrogen from residue Leu-141 that is likely to be destabilized or absent in unbound FVIIa is shown in lime.