FIGURE 10.

Binding of JAZ to the p21 promoter correlates with p21 expression during apoptosis. A, lysates from CGNs treated with HK or LK medium for 3, 6, and 9 h were used in Western blot analysis. The blots were probed sequentially with p21 and ERK antibodies. ERK serves as a loading control. B, quantification of p21 levels in CGNs treated with HK or LK for 3, 6, and 9 h and performed from three independent experiments. *, p < 0.05, 6 h LK-treated cells as compared with 6 h HK-treated cells. C, CGN cultures were either untreated (UNT) or treated with HK or LK medium for 3 and 6 h. ChIP assay was performed using IgG and JAZ antibodies for immunoprecipitation (IP). Primers to the promoter region of the rat p21 genes were used to amplify the input and pulldown DNA.