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. 2014 Oct 20;289(51):35409–35420. doi: 10.1074/jbc.M114.597575

FIGURE 12.

FIGURE 12.

JAZ protects against mutant huntingtin and mutant ataxin-1 neurotoxicity. A, CGNs were transfected with GFP and Htt-Q15-GFP or Htt-Q138-GFP alone or co-transfected with JAZ-FLAG for 8 h followed by treatment with HK medium for 24 h. Data represent the mean ± S.E. from four independent experiments. *, p < 0.05, Htt-Q138-transfected neurons as compared with control (GFP-transfected neurons in HK). **, p < 0.01, JAZ/Htt-Q138-transfected neurons as compared with Htt-Q138-transfected neurons. B, CGNs were transfected with GFP, JAZ-HA, and Atx1–30Q-FLAG or Atx1–82Q-FLAG alone or co-transfected with JAZ-HA for 24 h followed by a switch to HK medium for 24 h. Immunocytochemistry and viability assessment was performed as described previously. Data represent mean ± S.E. from four independent experiments. **, p < 0.01, Atx1–82Q-transfected neurons as compared with control (GFP-transfected neurons under HK conditions). ***, p < 0.001, JAZ/Atx1–82Q-transfected neurons as compared with Atx1–82Q-transfected neurons.