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. 2014 Oct 20;289(51):35409–35420. doi: 10.1074/jbc.M114.597575

FIGURE 3.

FIGURE 3.

Overexpression of JAZ protects neurons from death. A, CGN cultures were transfected with GFP or JAZ-FLAG and allowed to express for 8 h followed by a switch to HK or LK medium for 24 h. Immunocytochemistry was performed using either GFP or FLAG antibody. Data represent the mean ± S.E. from eight independent experiments. ***, p < 0.0001 FLAG-tagged JAZ-transfected neurons in LK as compared with GFP-transfected neurons in LK. B, CGNs were transfected with either GFP or JAZ-HA. Treatment, immunocytochemistry, and viability assay was performed as described previously. Data represent the mean ± S.E. from four independent experiments. ***, p < 0.0001, HA-tagged JAZ-transfected neurons in LK as compared with GFP-transfected neurons in LK. C, cortical neuron cultures transfected with GFP or JAZ-FLAG were treated with 1 mm HCA for 18 h. Data represent the mean ± S.E. from four independent experiments. *, p < 0.05, JAZ-transfected neurons treated with HCA as compared with GFP-transfected neurons treated with HCA. D, JAZ localizes inside the nucleus. CGNs transfected with JAZ-FLAG were switched to HK medium for 24 h, and immunocytochemistry was performed using FLAG antibody (top). CGN cultures were fixed, and immunocytochemistry was performed using JAZ antibody (bottom).