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. 2014 Oct 20;289(51):35409–35420. doi: 10.1074/jbc.M114.597575

FIGURE 6.

FIGURE 6.

JAZ kills cell line and inhibits cell cycle progression. HT22 (A) and HEK293T (B) cell lines were transfected with GFP or JAZ-FLAG for 24 h. Immunocytochemistry was performed using GFP or FLAG antibody. Data represent the mean ± S.E. for four independent experiments. ***, p < 0.0001, or **, p < 0.01, JAZ-transfected cells as compared with controls (GFP-transfected cells). C and D, BrdU assay of JAZ in HT22 (C) and HEK293T (D) cell lines. Both cell lines were transfected with GFP and JAZ-FLAG for 22 h, and BrdU was added in 20 μm concentration for 2 h. Immunocytochemistry was performed using BrdU and FLAG or GFP antibody. The proportion of transfected cells that were BrdU-positive was quantified. **, p < 0.01, JAZ-transfected/BrdU-positive cells as compared with GFP-transfected/BrdU-positive cells. E, representative picture of BrdU and JAZ or GFP transfected cells.