FIGURE 1.

Patch clamp speed. Peak-normalized current traces for the fast deactivation of TRPM8 at 30 °C, −260 mV (black), and the capacitive current in response to a −5 mV voltage step (red) are shown. It can be seen that there is substantial TRMP8 current when the capacitive current has vanished, allowing for a reliable measure of fast deactivation kinetics. Time constants for the fast component of TRPM8 deactivation currents and capacitive transient were 32 and 5.1 μs, respectively. Current signal was low-pass filtered at 40 kHz and sampled at 5 μs per point (200 kHz).