FIGURE 10.
Global fit of the data to the three-tiered allosteric model. A and B, Po versus voltage. C, τslow versus voltage, obtained from activation and deactivation kinetics. D, τfast versus voltage, obtained from deactivation kinetics only. Parameters: opening reaction, μ0 = 9.0 × 10−4 ms−1, ν0 = 3.6 ms−1 (M = 2.5 × 10−4), γ0 = 2.4 ms−1, δ0 = 157.2 ms−1 (L = 1.5 × 10−2); voltage sensor, V0 = 267 mV, z?J = 0.35; temperature sensor, ΔH = −29.8 kcal mol−1, ΔS = −104 cal mol−1 K−1 (To = 13.2 °C), voltage-coupled allosteric factors d1 = 6.6, d−1 = 0.86 (D = 7.7), f1 = 1.1, f−1 = 0.77 (F = 1.36); temperature-coupled allosteric factors c1 = 0.66, c−1 = 0.63 (C = 1.1), g1 = 0.68, g−1 = 0.18 (G = 3.8); interaction factor between voltage- and temperature- sensors, E = 29.6.