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. 2014 Oct 30;289(51):35456–35467. doi: 10.1074/jbc.M114.621599

FIGURE 8.

FIGURE 8.

Isoquercitrin suppresses cell proliferation in colon cancer cells but does not affect non-tumoral colon cells. Colon cancer cells were analyzed by Click-it EdU assay to measure proliferation. Click-it EdU immunocytochemistry is shown for SW480 (A–D), HCT116 (G–J), DLD-1 (M–P), and IEC-18 (S–V) cells. Isoquercitrin (Isoq.) treatment significantly reduced cell proliferation in all colon cancer cell lines analyzed, but did not affect cell proliferation in non-tumor cell line IEC-18. E, in SW480 cells, the proliferation ratio decreased nearly to 24%. F, L, and R, total number of cells in SW480, HCT116, and DLD-1 also decreased nearly to 50%. K, in HCT116 cells, the proliferation ratio decreased nearly to 18%. Q, in DLD-1 cells, the proliferation ratio decreased nearly to 17%. W and X, in IEC18 cells, the proliferation ratio was not affected (W), and the total number of cells was unaffected (X) by isoquercitrin treatment. *, p < 0.05; **, p < 0.01.