FIGURE 4.

I-BET151 acts downstream of Smo in the HH pathway. A, Sufu^−/− MEFs harbor a constitutively active HH signaling pathway, activated downstream of Smo. These MEFS were treated with either I-BET151 (100 nm or 500 nm) or vismodegib (100 nm) for 24 h, and the expression levels of various HH signaling components was determined. B, Sufu^−/− MEFs were treated with 0.5 μm I-BET151 for 8, 16, or 24 h. RNA was collected and subjected to quantitative RT-PCR. The expression of the indicated gene products was then normalized to that of Gapdh. Error bars represent the S.E. of three independent experiments. p values ≤ 0.05 are considered statistically significant and indicated by an asterisk.