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. 2014 Nov 4;289(51):35503–35516. doi: 10.1074/jbc.M114.590687

FIGURE 2.

FIGURE 2.

Hes3 is expressed in cultured MIN6 cells under defined conditions. A, cells propagate under all three culture conditions as shown by EdU incorporation (representative immunocytochemistry images shown; insets represent DAPI staining) (image width: 387 μm). B, DC promote nuclear Hes3 localization and increase nuclear Pdx1 localization. Nuclear Hes3 and Pdx1 co-localize. (representative immunocytochemistry images shown) (image widths: 450 μm). C, culture conditions affect gene expression profiles as revealed by Affymetrix DNA microarrays. D, culture conditions affect Pdx1 expression, and insulin content (Western blotting images shown); DC increase Pdx1 expression and insulin content; the effects are reversed by re-plating the cells in RC (GAPDH is used as the housekeeping gene). E, released insulin measurements by ELISA under the three different conditions show that the cells are responsive to glucose challenge in all conditions (n = 3; errors bars represent S.E. R, resting state; S, stimulated state). F, stimulation index measurements (expressed as % of the value in CC) by ELISA under the three different conditions show that the cells are responsive to glucose challenge in all conditions (n = 3; errors bars represent S.E.).