Skip to main content
. 2014 Nov 5;289(51):35517–35529. doi: 10.1074/jbc.M114.587709

FIGURE 5.

FIGURE 5.

X11α localizes to Golgi outposts in dendrites. A, relative co-localization of X11α with TGN38, a marker of the trans-Golgi network, in the soma of rat cultured cortical neurons (DIV 24). Arrows indicate co-localization, and arrowheads indicate X11α puncta that do not co-localize with TGN38. White dashed line denotes cell soma; red dashed line denotes cell nucleus. B, relative localization of X11α with giantin, a general marker for the Golgi complex. Dashed boxes indicate regions magnified in C. C, insets from B from proximal (panel i) and distal (panel ii) regions of the apical dendrite. Arrows indicate co-localization, and arrowheads indicate X11α puncta that do not co-localize with giantin. D, relative localization of endogenous X11α with overexpressed YFP-GalT in Golgi outposts in dendritic shaft. Dashed box indicates dendrite region magnified in E. E, magnified dendrite region from D. Arrows indicate co-localized puncta between YFP-GalT and X11α (overlay in white). Arrowheads indicate X11α puncta that do not co-localize with YFP-GalT. F, quantification of X11α co-localization with either giantin or YFP-GalT in dendrites. G, co-localization of X11α with giantin and YFP-GalT at dendrite branch points. Gray dashed lines outline the dendritic shaft. Scale bars, 1 μm (A); 5 μm (C, E, and G).