TABLE 3.
Antibodies used for experimental procedures
The rabbit polyclonal anti-PTF1a directed against the C-terminal KSFDNIENEPPFEFVS peptide of PTF1a is a custom antibody generated by Eurogentec.
| Antibodies against | Species | Usesa | Dilutions | Suppliers |
|---|---|---|---|---|
| PTF1a | Rabbit polyclonal | WB | 1:2000 | Eurogentec |
| IP | 10 μl/assay | |||
| IHC | 1:500 | |||
| TRIP12 | Rabbit polyclonal | IHC | 1:200 | Bethyl Laboratories |
| IP | 4 μg/500 μg of protein | |||
| Ubiquitin | Mouse monoclonal | WB | 1:2000 | Cell Signaling Technology |
| GFP | Mouse monoclonal | WB | 1:200 | Santa Cruz Biotechnology |
| IP | 4 μg/500 μg of protein | |||
| GAPDH | Rabbit polyclonal | WB | 1:1000 | Santa Cruz Biotechnology |
| HA | Rabbit monoclonal | WB | 1:1000 | Cell Signaling |
a WB, Western blot; IP, immunoprecipitation; IHC, immunohistochemistry.