FIGURE 1.

TGF-β1 represses TBX2 protein and mRNA expression. TBX2 protein from TGF-β1 (5 ng/ml) treated MCF-12A cells (A) or B16 cells (B) were prepared after indicated times and was examined by Western blot analysis. p38 was used as a loading control. Total RNA extracted from MCF-12A cells (C) or B16 cells (D) after indicated times with TGF-β1 treatment were reverse-transcribed and subjected to qRT-PCR using primers specific to TBX2. mRNA levels were normalized to GUSB. Bars, S.D. **, p < 0.001.