FIGURE 2.

TBX2 is transcriptionally regulated by TGF-β1. A, luciferase assay using MCF-12A cells, which were transfected with full-length human TBX2 promoter (400 ng) constructs and were treated with 5 ng/ml TGF-β1. Mean values (± S.D.) are presented as fold activity over that of an empty firefly luciferase reporter and are representative of at least three independent experiments. MCF-12A (B) and B16 (C) cells were pretreated with vehicle (control) or 30 μg/ml CHX for 1 h and treated with TGF-β1 for 8 h for MCF-12A cells and 4 h for B16 cells. RNA and protein were harvested for use in qRT-PCR and Western blotting analysis. The expression of TBX2 was quantified using UN-SCAN-IT gel 6.1 software and the densitometric values normalized to p38 levels. Bars, S.D. *, p < 0.05; **, p < 0.001.