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HSQC-DEPT (blue and green) and HMBC (red) spectra of native lipid A from B. japonicum. Lipid A sample was dissolved in CDCl3/CD3OD (2:1, v/v) with traces of D2O. The signals were marked as follows: A, α-d-GalpA; B, α-d-GlcpN3N; C, β-d-GlcpN3N; D, α-d-Manp; E, α-d-Manp; Hop-32 and Hop-33, CH-OH groups at positions of 32 and 33, respectively, of hopanoid lipid side chain (see Fig. 4).
