TABLE 2.

Annotation of mass spectrometric information (main signals) obtained for the O-deacylated and native lipid A samples from B. japonicum (see Fig. 2, A and B)

Form of lipid A	Measured mass	Type of molecule	Composition	Calculated monoisotopic mass
	Da			Da
Lipid A O-deacylated	530.4312	Hopanoid		530.4335
	1669.030	Lipid Atetra-acyl	2 × GlcpN3N	1669.0174
			2 × Manp
			1 × GalpA
			2 × 14:0 (3-OH), 2 × 12:0 (3-OH)
			-H2O
Lipid A native	760.4712	[Y]-ion type	1 × GlcpN3N	760.4726
			1 × GalpA
			1 × 14:0 (3-OH), 1 × 12:0 (3-OH)
	1008.907	Hopanoid	Hopanoid 530.4335 u	1008.9084
		+ VLCFA	1 × 32:0 (31-OH)
	1651.011	Lipid Atetra-acyl	2 × GlcpN3N	1651.0068
		-H2O	2 × Manp
			1 × GalpA
			2 × 14:0 (3-OH), 2 × 12:0 (3-OH)
			−2 × H2O
	2087.390	Lipid Apenta-acyl	2 × GlcpN3N	2087.4353
		-H2O	2 × Manp
			1 × GalpA
			2 × 14:0 (3-OH), 2 × 12:0 (3-OH)
			1 × 29:0 (28-OH)
			− 2 × H2O
	2583.951	Lipid Ahexa-acyl	2 × GlcpN3N	2583.920
			2 × Manp
			1 × GalpA
			2 × 14:0 (3-OH), 2 × 12:0 (3-OH)
			1 × 29:0 (28-OH), 1 × 32:0 (31-OH)
			-H2O
	3096.291	Lipid Ahexa-acyl	2 × GlcpN3N	3096.3428
		+ hopanoid	2 × Manp
			1 × GalpA
			2 × 14:0 (3-OH), 2 × 12:0 (3-OH)
			1 × 29:0 (28-OH), 1 × 32:0 (31-OH)
			1 × hopanoid 530.4335
			-H2O
	3650.833	Lipid Ahexa-acyl	2 × GlcpN3N	3650.8126
		+ (2 × hopanoid)	2 × Manp
			1 × GalpA
			2 × 14:0 (3-OH), 2 × 12:0 (3-OH)
			1 × 31:0 (30-OH), 1 × 32:0 (31-OH)
			1 × hopanoid 530.4335
			1 × hopanoid 544.4491
			-H2O