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. 2014 Dec 19;4:7554. doi: 10.1038/srep07554

Figure 5. Adult-born neurons lacking IL-17 exhibit increased intrinsic excitability and receive more excitatory synaptic activity.

Figure 5

(A). A representative cultured neuron from the adult DG of hippocampus (i.p. injected with Brdu for 4 days before cell dissociation) was recorded and microinjected with biocytin then immunostained with streptavidin conjugated antibody (green) and BrdU antibody (red). (B). Typical traces of action potential generation in response to 90 pA current injection (note the -60 mV holding potential and the time course and membrane potential calibration bars) show that deletion of IL-17 signaling causes increased neuronal excitability in BrdU+ primary cultured neuron from the adult DG of hippocampus. (C). Calculated input-output relationships for action potential spike activity (ordinate) as a function of currents injected (0-180 pA) in cultured BrdU+ cortical neurons prepared from the adult DG of hippocampus. Results show that deletion of IL-17 induces the increase of action potential numbers as compared to WT controls. (D–F). Deletion of IL-17 also increases the amplitude and frequency of mEPSCs recorded from cultured BrdU+ neurons from the DG of hippocampus as compared to those from WT controls. n = 8. Means ± SEM; *p < 0.05; **p < 0.01.