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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Nat Commun. 2014 Nov 27;5:5583. doi: 10.1038/ncomms6583

Figure 6. FGF regulation of HSC specification requires the Notch ligand Dlc.

Figure 6

(a–d) Expression of runx1 in wt and hsp70:dn-fgfr1 embryos injected with 100pg of dlc mRNA and uninjected controls. Embryos were heat-induced at 12 hpf. (e, f) Quantitation of defective embryos showing decreased expression of runx1 at 26 hpf (e; uninjected wt, n=61; dlc + wt, n=44; uninjected hsp70:dn-fgfr1, n=46; dlc + hsp70:dn-fgfr1, n=52) and cmyb+ cells at 32 hpf (f; uninjected wt, n=68; dlc + wt, n=56; uninjected hsp70:dn-fgfr1, n=58; dlc + hsp70:dn-fgfr1, n=46) using hsp70:dn-fgfr1 injected with dlc mRNA and followed by heat-shock at 12 hpf (mean ± s.e.m.; *P-value < 0.05, Student’s t-test). (g–j) runx1 expression at 26 hpf in hsp70:gal4; UAS:NICD-myc; hsp70:dn-fgfr1 heat-shocked at both 12 and 14 hpf. (k, l) NICD+ embryos were identified by immunostaining for the myc epitope tag, following WISH. (m) Percentage of embryos with a runx1 phenotype at 26 hpf in wt or FGF-blocked, with or without enforced NICD expression (NICD− wt, n=48; NICD+ wt, n=25; NICD− hsp70:dn-fgfr1, n=20; NICD+ hsp70:dn-fgfr1, n=21). Red arrowheads indicate runx1 expression in the DA. Scale bars=100μm.