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. 2014 Dec 14;11:74. doi: 10.1186/s12989-014-0074-0

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© Pierdominici et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Flow cytometry immunophenotyping of DEP-treated T lymphocytes. Flow cytometry analysis of T cell activation markers (A) and cytokine expression at the single cell level (B) carried out in CD4⁺ and CD8⁺ T lymphocytes from 15 healthy donors after treatment with 30 μg/ml of E4 or E5 particles for 48 h (activation markers) or 72 h (cytokine production). For CD4⁺ and CD8⁺ T lymphocyte subsets, data were expressed as the percentage of each subset within the CD4⁺ or CD8⁺ population considered as 100%. Data are represented as box plots displaying medians, 25^th and 75^th percentiles as boxes, and 10^th and 90^th percentiles as whiskers. *p < 0.05 versus untreated cells.