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. 2014 Dec 19;5:270. doi: 10.3389/fphar.2014.00270

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Copyright © 2014 Ramachandra, Shaw, Shangaris, Loukogeorgakis, Guillot, De Coppi and David.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In utero autologous intraperitoneal transplantation of sheep amniotic fluid CD34⁺ cells in fetal sheep with long-term follow up. Engraftment in the peripheral blood after in utero transplantation of autologous sheep CD34⁺eGFP⁺ AF cells. All five born lambs showed eGFP⁺ cells in the peripheral blood at birth (M1-A, M2-A, M3-A, M2-B, and M3-B), and all three survivors revealed persistent levels of engraftment of around 2% that persisted up to the last sampling point at 6 months of age (M1-A, M2-B, and M3-A). Negative control: peripheral blood from uninjected sheep. M1, M2, and M3: the three ewes that showed negativity for eGFP signal.