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. 2014 Dec 19;5:270. doi: 10.3389/fphar.2014.00270

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Copyright © 2014 Ramachandra, Shaw, Shangaris, Loukogeorgakis, Guillot, De Coppi and David.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence for CK18 and AFP expression in fetal liver and amniotic fluid stem cells. Panel (A) shows positive CK18 expression in cells cultured from a control fetal sheep liver but no expression in amniotic fluid mesenchymal stem cells (AFMSCs) or transduced cells (GFP-AFMSCs) before injection. An uninjected sheep fetus of comparable gestational age was used as the control. Panel (B) shows co-expression of GFP with expression of Alpha fetoprotein (AFP) another liver specific marker in the fetal liver after transplantation of transduced AFMSCs. Scale bars: 20 µm.