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. 2014 Dec 19;5:650. doi: 10.3389/fimmu.2014.00650

Figure 5.

Figure 5

Vδ2-matured DC and B cells stimulate proliferation of resting allogeneic T cells. DC or B cells were co-cultured with HMB-PP-expanded human Vδ2 T cells in the absence or presence of HMB-PP (denoted H). After 24 h CellTrace-labeled allogeneic resting αβ T cells were added at a ratio of 10:1 and cultured for 6 days. (A) Representative dot plot showing proliferating T cells. (B) Histogram showing proliferating T cells (green peaks) versus unstimulated T cells (red peak) by flow cytometric analysis of cell trace dilution. (C) Average (±SEM) percentage of proliferating T cells when cultured with Vδ2-matured DC (n = 10; left) and levels of IFN-γ and IL-4 secreted by cultures of Vδ2 T cell matured DC with αβ T cells (n = 6–10). (D) Average (±SEM) percentage of proliferating T cells when cultured with Vδ2-matured B cells (n = 4) and levels of IFN-γ and IL-4 secreted by cultures of Vδ2 T cell matured DC with αβ T cells (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001, paired t-test versus T cells except where indicated by horizontal lines.