Table 1.
Ingredient | Control diet |
1% Sutherlandia diet |
2% Elderberry diet |
|||
---|---|---|---|---|---|---|
g/kg of diet | % of total | g/kg of diet | % of total | g/kg of diet | % of total | |
Cornstarch | 397.3 | 39.73 | 387.3 | 38.73 | 387.3 | 37.73 |
Casein | 200.0 | 20.00 | 200.0 | 20.00 | 200.0 | 20.00 |
Dextrose | 132.0 | 13.20 | 132.0 | 13.20 | 132.0 | 13.20 |
Sucrose | 100.0 | 20.00 | 100.0 | 10.00 | 100.0 | 10.00 |
Fiber (cellulose) | 50.0 | 5.00 | 50.0 | 5.00 | 50.0 | 5.00 |
Mineral mix (AIN-93) | 35.0 | 3.50 | 35.0 | 3.50 | 35.0 | 3.50 |
Vitamin mix (AIN-93G) | 10.0 | 1.00 | 10.0 | 1.00 | 10.0 | 1.00 |
l-cystine | 3.0 | 0.30 | 3.0 | 0.30 | 3.0 | 0.30 |
Choline bitartrate | 2.5 | 0.25 | 2.5 | 0.25 | 2.5 | 0.25 |
Soybean oil | 70.0 | 7.00 | 70.0 | 7.00 | 70.0 | 7.00 |
Food dye (color varies) | 0.2 | 0.02 | 0.2 | 0.02 | 0.2 | 0.02 |
Elderberry (whole ripe fruit)a | – | – | – | – | 20 | 2.00 |
Sutherlandia (dried leaves)b | – | – | 10 | 1.00 | – | – |
Total | 1,000 | 100 | 1,000 | 100 | 1,000 | 100 |
York and Bob Gordon cultivars (60:40 ratio) were harvested at the peak of ripeness, freeze-dried, then ground into a fine powder, and mixed with an equal weight of corn starch to prevent clumping. The amount of this mix added to the diet was actually twice that shown in the table.
Sutherlandia was sourced Big Tree Nutraceutical (21 First Avenue, Fish Hoek 7975, South Africa). Prior to its incorporation into the diet, the chopped leaves were grounded into a fine powder using a handheld coffee bean grinder.