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. 2014 Dec 11;44(1):134–143. doi: 10.1097/MPA.0000000000000223

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Copyright © 2014 by Lippincott Williams & Wilkins

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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Induced differentiation test of PC12 cells. A, PC12 cell for blank control was undifferentiated and fully seeded in 6-well plates on the 10th day. B, PC12 cells cultured with supernatant of Artemin-overexpressed PANC-1 cells stopped growth and showed significant neuronlike differentiation. C, PC12 cells cultured with supernatant of the empty vector pCDNA3.1 control cells showed a few neuronlike differentiation cells. D, PC12 cells cultured with supernatant of Artemin-depleted PANC-1 cells showed inconspicuous neuronlike differentiation. E, PC12 cells cultured with supernatant of the empty vector pCDNA6.2 control showed a few neuronlike differentiation cells.