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Generation and characterization of CD200-modified surfaces. (a) Schematic of construct used for soluble expression of extracellular CD200 protein (top). Coomassie-blue stained SDS-PAGE (left) and native PAGE (middle) gels, and Western blot analysis (right) of purified recombinant CD200 product (bottom). (b) Binding profile of CD200 immobilized on the streptavidin-coated polystyrene plate, measured with PE-labeled anti-mCD200.
