Table 1. Primer sequences used for qPCR analysis.
| Gene | Accession No. | Forward (F) and Reverse (R) Primers | Exon F, R | Gene Function |
| POLR2A | NM_000937 | F:AAGTTCAACCAAGCCATTGCG R:GACACACCAGCATAGTGGAAGG | 19–20, 20 | mRNA synthesis |
| TMEM222 | NM_032125 | F:TCTACGGGAAGTACGTCAGC R:CCATCACCGGAGGTTAAAGACC | 2–3, 3 | membrane integrity |
| MVK | NM_000431 | F:AAGGTAGCACTGGCTGTATCC R:CCAATGTTGGGTAAGCTGAGG | 6–8, 8 | cholesterol synthesis |
| DEFB1 | NM_005218 | F:GTTCCTGAAATCCTGGGTGTTG R:CTGTGAGAAAGTTACCACCTGAG | 1, 1–2 | immune response |
| PPIA | NM_021130 | F:GCTTGCTGGCAGTTAGATGTC R:AGAGGTCTGTTAAGGTGGGC | 5, 5 | protein folding |
| GAPDH | NM_002046 | F:ATTTGGCTACAGCAACAGGG R:TCAAGGGGTCTACATGGCA | 10, 10 | glycolytic enzyme |
| RPLP0 | NM_001002 | F:ACAATGGCAGCATCTACA R:GTAATCCGTCTCCACAGA | 6, 7 | protein synthesis |
| PGK1 | NM_000291 | F:GAGATGATTATTGGTGGTGGAA R:AGTCAACAGGCAAGGTAATC | 7, 8 | glycolytic enzyme |
| DICER1 | NM_177438 | F:GTACGACTACCACAAGTACTTC R:ATAGTACACCTGCCAGACTGT | 24, 25 | small RNA synthesis |
| DROSHA | NM_013235 | F:GCAGCGCAAAGGCAAGACGC R:AGGCGGGGAGACTGTGATCCG | 10, 11 | small RNA synthesis |
| ACTB | NM_001101.3 | F:CACACAGGGGAGGTGATAGC R:GACCAAAAGCCTTCATACATCTCA | 6, 6 | cellular structure |
POLR2A - polymerase (RNA) II (DNA directed) polypeptide A; TMEM222 - transmembrane protein 222; MVK - mevalonate kinase; DEFB1 - defensin beta 1; PPIA - peptidylprolyl isomerase; GAPDH - glyceraldehyde-3-phosphate dehydrogenase; RPLP0 - ribosomal protein, large, P0; PGK1 - phosphoglycerate kinase 1; DICER1 - dicer 1, ribonuclease type III; DROSHA - drosha, ribonuclease type III; ACTB – actin, beta.