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| Primer | Linear range (Cq) | r2 | Efficiency |
| GAPDH | 9 to 27 | 0.9997 | 98% |
| PGK1 | 11 to 29 | 0.9981 | 91% |
| PPIA | 10 to 27 | 0.9998 | 104% |
| RPLP0 | 11 to 30 | 0.9992 | 93% |
| TMEM222 | 10 to 36 | 0.9998 | 93% |
| POLR2A | 11 to 29 | 0.9998 | 98% |
| MVK | 10 to 28 | 0.9997 | 96% |
| DEFB1 | 10 to 32 | 0.9998 | 99% |
| ACTB | 10 to 32 | 0.9995 | 89% |
| DICER1 | 9 to 32 | 0.9994 | 100% |
| DROSHA | 10 to 34 | 0.9983 | 99% |
