Table 3. Summary of key statistics after BestKeeper analysis.
Colonic HT-29 cells | ||||||
DICER1 | MVK | PGK1 | POLR2A | PPIA | RPLP0 | |
s.d. [±Cq] | 0.68 | 0.71 | 0.95 | 0.97 | 0.67 | 0.88 |
coeff. of corr. [r] | 0.947 | 0.823 | 0.988 | 0.931 | 0.935 | 0.877 |
p-value | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 |
Power [x-fold] | 1.77 | 1.79 | 2.23 | 2.30 | 1.72 | 1.97 |
The standard deviation (s.d.) corresponds to the individual gene stability and the coefficient of correlation, with its respective p-value, corresponds to how closely the candidate reference gene resembles the BestKeeper ideal normalisation factor after repeated pair-wise analysis. The power value is determined by regression analysis, using fold change (x-fold) as a reference point, and a smaller value indicates a better reference candidate. Values for genes eliminated in earlier stages of analysis are not shown.