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. 2014 Dec 19;9(12):e115592. doi: 10.1371/journal.pone.0115592

Table 3. Summary of key statistics after BestKeeper analysis.

Colonic HT-29 cells
DICER1 MVK PGK1 POLR2A PPIA RPLP0
s.d. [±Cq] 0.68 0.71 0.95 0.97 0.67 0.88
coeff. of corr. [r] 0.947 0.823 0.988 0.931 0.935 0.877
p-value 0.001 0.001 0.001 0.001 0.001 0.001
Power [x-fold] 1.77 1.79 2.23 2.30 1.72 1.97

The standard deviation (s.d.) corresponds to the individual gene stability and the coefficient of correlation, with its respective p-value, corresponds to how closely the candidate reference gene resembles the BestKeeper ideal normalisation factor after repeated pair-wise analysis. The power value is determined by regression analysis, using fold change (x-fold) as a reference point, and a smaller value indicates a better reference candidate. Values for genes eliminated in earlier stages of analysis are not shown.