Table 5. Comprehensive ranking of reference gene candidates by calculation of a geometric mean.
| Colonic HT-29 cells | |||||
| geNorm | BestKeeper | NormFinder | ΔCq | Ranking | Mean |
| PGK1 | PGK1 | PGK1 | PGK1 | PGK1 | 1.000 |
| RPLP0 | DICER1 | DICER1 | DICER1 | DICER1 | 2.378 |
| PPIA | POLR2A | PPIA | PPIA | PPIA | 3.224 |
| DICER1 | PPIA | POLR2A | GAPDH | RPLP0 | 4.356 |
| GAPDH | RPLP0 | MVK | POLR2A | POLR2A | 4.681 |
| DROSHA | MVK | RPLP0 | RPLP0 | GAPDH | 5.595 |
| MVK | GAPDH | GAPDH | MVK | DROSHA | 6.055 |
| POLR2A | DROSHA | DROSHA | DROSHA | MVK | 6.192 |
| TMEM222 | TMEM222 | TMEM222 | TMEM222 | TMEM222 | 9.000 |
| ACTB | ACTB | ACTB | ACTB | ACTB | 10.000 |
| DEFB1 | DEFB1 | DEFB1 | DEFB1 | DEFB1 | 11.000 |