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. 2014 Dec 19;9(12):e115273. doi: 10.1371/journal.pone.0115273

Figure 3. The block of P2Y1R counteracts the synaptic failure induced by severe OGD in the DG.

Figure 3

AD was recorded as a negative d.c. shift in response to 9-min OGD in control (a, n = 26). MRS2179 (10 µM) completely prevented the appearance of AD in 23 out of 28 slices during 9-min OGD (b). MRS2179 (10 µM) had no effect in 5 out of 28 slices after 9-min OGD (c, n = 6), in which AD was recorded as a negative d.c. shift. d. example traces taken at the time-points indicated on the graph, immediately before (1), 10 min after the beginning of MRS2179 application (2), 9 minutes after OGD+MRS2179 (3) or 50 min after washout in oxygenated aCSF (4). Inset: example trace taken from the same hippocampal slice 24 hours from the end of OGD applied in the presence of 10 µM MRS2179. In each graph, traces are averages of 3 consecutive responses. Scale bars: 10 ms, 0.5 mV. f. The graph shows the time-course of the effect caused by 9-min OGD on fEPSP amplitude (mean±SEM) in untreated OGD slices (n = 26) and in OGD slices treated with 10 µM MRS2179, in which AD was absent (n = 23) or present (n = 5). fEPSPs amplitude is expressed as percent of the respective pre-ischemic baseline. Open bar: time of drug application.