Table 3. Assay precision, ranges, lower limits of detection (LOD) and assay linearity for MMAT.
| Analyte and Cutoff Value | LOD | Control | Intra-assay precision (mean, CV) | Inter-assay precision (mean, CV) | Assay Linearity | |
| AGP (µg/mL) (>1000 µg/mL indicates inflammation) [33] | 2.98 | High | 450.7, 3.0% | 460.9, 7.9% | 1∶2 | 87% |
| Medium | 114.1, 0.6% | 113.7, 11.2% | 1∶4 | 99% | ||
| Low | 27.3, 6.5% | 29.2, 13.9% | 1∶8 | 98% | ||
| 1∶16 | 101% | |||||
| CRP (µg/mL) (>5 µg/mL indicates inflammation) [33] | 0.41 | High | 52.3, 4.9% | 50.3, 8.8% | 1∶2 | 101% |
| Medium | 12.1, 0.6% | 12.2, 5.6% | 1∶4 | 100% | ||
| Low | 3.4, 4.2% | 3.4, 9.3% | 1∶8 | 92% | ||
| 1∶16 | 89% | |||||
| Ferritin (ng/mL) ID cutoff <15 ng/mL (under 5 years, <12 ng/mL) [33] | 1.5 | High | 279.4, 4.1% | 279.4, 9.3% | 1∶2 | 117% |
| Medium | 70.1, 6.1% | 70.1, 12.8% | 1∶4 | 85% | ||
| Low | 31.5, 14.8% | 31.5, 14.1% | 1∶8 | 111% | ||
| 1∶16 | 114% | |||||
| RBP4 (µg/mL) VAD cutoff <14.7 µg/mL (0.7 µmol/L) [41] | 0.89 | High | 41.3, 11.3% | 42.4, 9.3% | 1∶2 | 96% |
| Medium | 10.7, 0.7% | 10.8, 8.7% | 1∶4 | 109% | ||
| Low | 3.2, 2.2% | 3.0, 5.3% | 1∶8 | 107% | ||
| 1∶16 | 97% | |||||
| sTfR (µg/mL) ID cutoff >8.3 µg/mL [34] | 0.08 | High | 3.6, 2.0% | 3.4, 7.3% | 1∶2 | 110% |
| Medium | 0.9, 0.3% | 0.9, 9.3% | 1∶4 | 98% | ||
| Low | 0.2, 11.1% | 0.2, 13.5% | 1∶8 | 91% | ||
| 1∶16 | 80% | |||||
AGP, alpha-1-acid glycoprotein; CRP, C-reactive protein; RBP, retinol binding protein 4; sTfR, soluble transferrin receptor; ID, iron deficiency; VAD, vitamin A deficiency; MMAT, multiplexed micronutrient assessment tool; CV, coefficient of variation.