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. 2014 Dec 19;9(12):e114576. doi: 10.1371/journal.pone.0114576

Figure 1. Phylogenetic relationships among 30 V. vulnificus strains inferred from GoldenGate genotyping results compared to sequencing.

Figure 1

Results were based on Illuminus-P genotype calls obtained by the GoldenGate assay at 31 SNPs compared to sequencing analysis of the same 12 intergenic loci analyzed as sequence types. These loci served for array quality control. A nonparametric analysis of allelic variation was used to calculate the Nei coefficient of association and to generate the corresponding matrix with SAS 8.02, followed by minimum-evolution (ME) cluster analysis using MEGA5.