Fig. 5.

Immunoblotting of E-NTPDases in fractionated plasma. a Immunoblots of the fractionated plasma using E-NTPDase antibodies. Samples of the first fraction from the size exclusion column were separated by non-reducing SDS-PAGE and then probed with antibodies as indicated. Molecular weight standards are shown in the right lane. b Representative immunoblots of microparticles from human plasma (3 of the 14 samples were shown). Microparticles from human subjects were prepared by differential ultracentrifugation and then analyzed by immunoblotting