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. 2014 Oct 24;156(1):323–333. doi: 10.1210/en.2014-1296

Figure 2. CTNNB1 potentiates activin-stimulated Fshb expression in gonadotrope cells.

Figure 2.

Cells were cultured from pituitary glands of male Δex3-GRIC or control mice and treated or not with 1nM activin A. After 24 hours, cells were harvested for analysis of Fshb or Lhb mRNA levels by real-time RT-PCR (A), and spent media were collected for analysis of FSH and LH (B). Experiments were performed 3 times, in triplicate. Columns, means of the 3 experimental replicates; error bars, SEM. Columns labeled with different letters were statistically different (P < .05, two-way ANOVA with Tukey's post hoc test).