Figure 3. CTNNB1 potentiates GnRH- and activin-stimulated Fshb promoter activity in LβT2 cells.

LβT2 cells were transfected with the indicate murine Fshb promoter luciferase construct and an expression vector encoding wild-type (A) or Δex3 mutant (B) CTNNB1, or the empty pcDNA 3.0 vector as a control, before treatment with GnRH (100nM), activin (1nM), neither, or both. Luciferase activity values are expressed relative to the empty vector/no ligand control. Experiments were performed 3 times, in triplicate. Columns, means of the 3 experimental replicates; error bars, SEM. Columns labeled with different letters were statistically different (P < .05, three-way ANOVA with Tukey's post hoc test).