Figure 1. Sortilin is expressed at low levels in castration-resistant cells expressing progranulin and sortilin expression levels regulates motility.

A, A total of 40 μg of protein lysates from the indicated cell lines were analyzed for sortilin expression by immunoblot with anti-sortilin monoclonal antibodies. B and C, Progranulin expression in lysates and conditioned medium (CM) of various cell lines was detected by immunoblot as described (6, 7). Immunoblot for β-actin was used as loading control. Blots are representative of 2 independent experiments. D, PC3 cells were transfected with the human Sortilin vector and vector control as described in Materials and Methods. Sortilin expression was tested by immunoblot with anti-sortilin monoclonal antibodies. E, Progranulin expression in various PC3 cell lines was analyzed by immunoblot in lysates and CMs normalized over number of cells as previously described (6, 7). Blots are representative of 2 independent experiments. Densitometric analysis is expressed as arbitrary units. F, Migration was performed as described in Materials and Methods. Data are the average of 3 independent experiments run in triplicates ± SD. ***, P < .001.