Skip to main content
. 2014 Nov 3;156(1):58–70. doi: 10.1210/en.2014-1590

Figure 3. Progranulin depletion in PC3 cells inhibits motility, invasion, proliferation, and anchorage-independent growth.

Figure 3.

A, PC3 cells, stably depleted of endogenous progranulin, were generated by transfecting the pRS-shRNA-control (scrambled shRNA) and pRS/shPGRN plasmids. Cells were selected in medium supplemented with 2 μg/mL of puromycin. After selection, pools of progranulin-depleted PC3 cells were tested for progranulin expression levels in cell lysates and conditioned media by immunoblot using anti-progranulin polyclonal antibodies as previously described (6, 7). Blots are representative of 2 independent experiments. Densitometric analysis is expressed as arbitrary units. Migration (B), wound healing (C), invasion (D), proliferation (E), and anchorage-independent growth (F) in soft-agar were performed as described in Materials and Methods. In soft-agar assays, only colonies of more than 150 μm were counted. Data are the average of 3 independent experiments run in triplicates ± SD. ***, P < .001.